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vectashild mounting medium with dapi  (Vector Laboratories)


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    Structured Review

    Vector Laboratories vectashild mounting medium with dapi
    Vectashild Mounting Medium With Dapi, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 98/100, based on 21970 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/vectashild+with+dapi/VECTASHIELD+Antifade+Mounting+Medium+with+DAPI/pm40191989-54-17-22
    Average 98 stars, based on 21970 article reviews
    vectashild mounting medium with dapi - by Bioz Stars, 2026-09
    98/100 stars

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    other:

    Article Title: Methods and compositions for the efficient delivery of therapeutic agents to cells and animals
    Article Snippet: Sections of 5 μm were fixed with in 100% acetone at 4° C. for 10 min, air-dried and mounted in Vectashild with DAPI (Vector Laboratories, Inc. Burlingame, Calif.).

    Article Title: Forced expression of Nanog in hematopoietic stem cells results in a gammadeltaT-cell disorder.
    Article Snippet: Sections were mounted with VECTASHILD with DAPI (Vector Laboratories, Burlingame, CA).



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    IL-7 co-localizes with the recycling endosome marker, Rab11, in cytokine pulsed lymphocytes. Cytospin preparations of PBMC were immunostained for IL-7 and Rab11 after 1 hour of incubation with rIL-7 (100 ng/ml) as described in the methods. Representative image of one lymphocyte showing (A) IL-7 (red), (B) Rab11 (green), and (C) <t>DAPI</t> (blue). (D) Merged image indicates evidence of co-localization <t>of</t> <t>fluorescent</t> signals. (E) The correlation coefficients (Pearson’s R value, and Spearman’s rank correlation value(ρ) for pixel co-localization of IL-7 and Rab11 signal in 18 cells is shown and plotted from the cell with the least correlation to the cell with the greatest correlation. Cells that were not pulsed with IL-7 and antibody isotype control stains were used as negative controls and did not demonstrate evidence of staining (not shown).
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    IL-7 co-localizes with the recycling endosome marker, Rab11, in cytokine pulsed lymphocytes. Cytospin preparations of PBMC were immunostained for IL-7 and Rab11 after 1 hour of incubation with rIL-7 (100 ng/ml) as described in the methods. Representative image of one lymphocyte showing (A) IL-7 (red), (B) Rab11 (green), and (C) <t>DAPI</t> (blue). (D) Merged image indicates evidence of co-localization <t>of</t> <t>fluorescent</t> signals. (E) The correlation coefficients (Pearson’s R value, and Spearman’s rank correlation value(ρ) for pixel co-localization of IL-7 and Rab11 signal in 18 cells is shown and plotted from the cell with the least correlation to the cell with the greatest correlation. Cells that were not pulsed with IL-7 and antibody isotype control stains were used as negative controls and did not demonstrate evidence of staining (not shown).
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    IL-7 co-localizes with the recycling endosome marker, Rab11, in cytokine pulsed lymphocytes. Cytospin preparations of PBMC were immunostained for IL-7 and Rab11 after 1 hour of incubation with rIL-7 (100 ng/ml) as described in the methods. Representative image of one lymphocyte showing (A) IL-7 (red), (B) Rab11 (green), and (C) <t>DAPI</t> (blue). (D) Merged image indicates evidence of co-localization <t>of</t> <t>fluorescent</t> signals. (E) The correlation coefficients (Pearson’s R value, and Spearman’s rank correlation value(ρ) for pixel co-localization of IL-7 and Rab11 signal in 18 cells is shown and plotted from the cell with the least correlation to the cell with the greatest correlation. Cells that were not pulsed with IL-7 and antibody isotype control stains were used as negative controls and did not demonstrate evidence of staining (not shown).
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    Image Search Results


    IL-7 co-localizes with the recycling endosome marker, Rab11, in cytokine pulsed lymphocytes. Cytospin preparations of PBMC were immunostained for IL-7 and Rab11 after 1 hour of incubation with rIL-7 (100 ng/ml) as described in the methods. Representative image of one lymphocyte showing (A) IL-7 (red), (B) Rab11 (green), and (C) DAPI (blue). (D) Merged image indicates evidence of co-localization of fluorescent signals. (E) The correlation coefficients (Pearson’s R value, and Spearman’s rank correlation value(ρ) for pixel co-localization of IL-7 and Rab11 signal in 18 cells is shown and plotted from the cell with the least correlation to the cell with the greatest correlation. Cells that were not pulsed with IL-7 and antibody isotype control stains were used as negative controls and did not demonstrate evidence of staining (not shown).

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    Article Title: Recycled interleukin-7 can be delivered to neighboring T cells

    doi: 10.4049/jimmunol.1400560

    Figure Lengend Snippet: IL-7 co-localizes with the recycling endosome marker, Rab11, in cytokine pulsed lymphocytes. Cytospin preparations of PBMC were immunostained for IL-7 and Rab11 after 1 hour of incubation with rIL-7 (100 ng/ml) as described in the methods. Representative image of one lymphocyte showing (A) IL-7 (red), (B) Rab11 (green), and (C) DAPI (blue). (D) Merged image indicates evidence of co-localization of fluorescent signals. (E) The correlation coefficients (Pearson’s R value, and Spearman’s rank correlation value(ρ) for pixel co-localization of IL-7 and Rab11 signal in 18 cells is shown and plotted from the cell with the least correlation to the cell with the greatest correlation. Cells that were not pulsed with IL-7 and antibody isotype control stains were used as negative controls and did not demonstrate evidence of staining (not shown).

    Article Snippet: Next, the slides were hard-mounted in Vectashild™ with DAPI and examined by an automated epi-fluorescent microscope (EVOS ® FL, Life Technologies).

    Techniques: Marker, Incubation, Staining